Yesterday last thing I set up some SP2 myeloma cells before going home.
I grabbed a convenient bottle of 'normal' culture medium that had been prepared for a different project and wasn't used.
Said medium contains HAT for hybridoma culture.
I was un-aware of the HAT content.
Said SP2 cells are specially engineered to find HAT lethal.
It is good to know that this system works well. I shall be quite confident that any cells growing after my fusion on Christmas eve will, absolutely definitely be hybridomas and not suriving myelomas.
Now I DO regret making Chris wait 5 more minutes to collect me.